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Ghrelin Regulates Glucose and Glutamate Transporters in Hypothalamic Astrocytes

Författare och institution:
E. Fuente-Martin (-); C. Garcia-Caceres (-); P. Argente-Arizon (-); F. Diaz (-); M. Granado (-); A. Freire-Regatillo (-); D. Castro-Gonzalez (-); M. L. Ceballos (-); L. M. Frago (-); Suzanne L. Dickson (Institutionen för neurovetenskap och fysiologi, sektionen för fysiologi); J. Argente (-); J. A. Chowen (-)
Publicerad i:
Scientific Reports, 6 s. Article number: 23673
ISSN:
2045-2322
Publikationstyp:
Artikel, refereegranskad vetenskaplig
Publiceringsår:
2016
Språk:
engelska
Fulltextlänk:
Sammanfattning (abstract):
Hypothalamic astrocytes can respond to metabolic signals, such as leptin and insulin, to modulate adjacent neuronal circuits and systemic metabolism. Ghrelin regulates appetite, adiposity and glucose metabolism, but little is known regarding the response of astrocytes to this orexigenic hormone. We have used both in vivo and in vitro approaches to demonstrate that acylated ghrelin (acyl-ghrelin) rapidly stimulates glutamate transporter expression and glutamate uptake by astrocytes. Moreover, acyl-ghrelin rapidly reduces glucose transporter (GLUT) 2 levels and glucose uptake by these glial cells. Glutamine synthetase and lactate dehydrogenase decrease, while glycogen phosphorylase and lactate transporters increase in response to acyl-ghrelin, suggesting a change in glutamate and glucose metabolism, as well as glycogen storage by astrocytes. These effects are partially mediated through ghrelin receptor 1A (GHSR-1A) as astrocytes do not respond equally to desacyl-ghrelin, an isoform that does not activate GHSR-1A. Moreover, primary astrocyte cultures from GHSR-1A knock-out mice do not change glutamate transporter or GLUT2 levels in response to acyl-ghrelin. Our results indicate that acyl-ghrelin may mediate part of its metabolic actions through modulation of hypothalamic astrocytes and that this effect could involve astrocyte mediated changes in local glucose and glutamate metabolism that alter the signals/nutrients reaching neighboring neurons.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
MEDICIN OCH HÄLSOVETENSKAP ->
Klinisk medicin
Nyckelord:
body-weight, food-intake, differential expression, primary cultures, arcuate nucleus, brain, neurons, obesity, metabolism, activation
Postens nummer:
236982
Posten skapad:
2016-05-27 13:00
Posten ändrad:
2016-05-31 14:12

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