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Göteborgs universitets publikationer

Fluorescence enhancement from single DNA molecules confined in SiO 2 nanochannels

Författare och institution:
Fredrik Westerlund (Institutionen för fysik (GU) & Institutionen för kemi- och bioteknik, Fysikalisk kemi, Chalmers); Fredrik Persson (Institutionen för fysik (GU)); A. Kristensen (-); Jonas O. Tegenfeldt (Institutionen för fysik (GU))
Publicerad i:
14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010; Groningen; Netherlands; 3 October 2010 through 7 October 2010, 1 s. 578-580
ISBN:
9781618390622
Publikationstyp:
Konferensbidrag, refereegranskat
Publiceringsår:
2010
Språk:
engelska
Sammanfattning (abstract):
We demonstrate that the detected emission intensity from YOYO-labeled DNA molecules confined in 180 nm deep Si/SiO2 nanofunnels changes significantly and not monotonically with the width of the funnel, an emission enhancement that is only detected for emitted light polarized parallel to the channel. We explain the enhancement effect as being due to optical phenomena in the channels. The enhancement effect may be of importance for quantitative fluorescence microscopy and for experiments with a tight photon budget.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
NATURVETENSKAP ->
Fysik
Nyckelord:
DNA, Fluorescence microscopy, Nanofluidics, Single molecule, Emission enhancement, Emission intensity, Enhancement effects, Fluorescence enhancement, Optical phenomena, Quantitative fluorescence microscopy, Single DNA molecules, Molecules
Postens nummer:
236436
Posten skapad:
2016-05-12 14:19

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