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Peptide Fragmentation and Surface Structural Analysis by Means of ToF-SIMS Using Large Cluster Ion Sources

Författare och institution:
Y. Yokoyama (-); S. Aoyagi (-); M. Fujii (-); J. Matsuo (-); John S. Fletcher (Institutionen för kemi och molekylärbiologi); N. P. Lockyer (-); J. C. Vickerman (-); M. K. Passarelli (-); R. Havelund (-); M. P. Seah (-)
Publicerad i:
Analytical Chemistry, 88 ( 7 ) s. 3592-3597
ISSN:
0003-2700
Publikationstyp:
Artikel, refereegranskad vetenskaplig
Publiceringsår:
2016
Språk:
engelska
Fulltextlänk:
Sammanfattning (abstract):
Peptide or protein structural analysis is crucial for the evaluation of biochips and biodevices, therefore an analytical technique with the ability to detect and identify protein and peptide species directly from surfaces with high lateral resolution is required. In this report, the efficacy of ToF-SIMS to analyze and identify proteins directly from surfaces is evaluated. Although the physics governing the SIMS bombardment process precludes the ability for researchers to detect intact protein or larger peptides of greater than a few thousand mass unit directly, it is possible to obtain information on the partial structures of peptides or proteins using low energy per atom argon duster ion beams. Large cluster ion beams, such as Ar dusters and C-60 ion beams, produce spectra similar to those generated by tandem MS. The SIMS bombardment process also produces peptide fragment ions not detected by conventional MS/MS techniques. In order to clarify appropriate measurement conditions for peptide structural analysis, peptide fragmentation dependency on the energy of a primary ion beam and ToF-SIMS specific fragment ions are evaluated. It was found that the energy range approximately 6 <= E/n <= 10 eV/atom is most effective for peptide analysis based on peptide fragments and [M + H] ions. We also observed the cleaving of side chain moieties at extremely low-energy E/n <= 4 eV/atom.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
NATURVETENSKAP ->
Kemi ->
Analytisk kemi
Nyckelord:
mass-spectrometry, universal equation, ar cluster, argon, beams, energy, yields, size, Chemistry
Postens nummer:
235542
Posten skapad:
2016-04-29 10:24
Posten ändrad:
2016-04-29 10:24

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