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Impact of ocean acidification on antimicrobial activity in gills of the blue mussel (Mytilus edulis)

Författare och institution:
Bodil Hernroth (-); Susanne P. Baden (Institutionen för biologi och miljövetenskap); H. Tassidis (-); K. Hörnaeus (-); J. Guillemant (-); SB Lind (-); Jonas Bergqvist (-)
Publicerad i:
Fish and Shellfish Immunology, 55 s. 452–459
ISSN:
1050-4648
E-ISSN:
1095-9947
Publikationstyp:
Artikel, refereegranskad vetenskaplig
Publiceringsår:
2016
Språk:
engelska
Fulltextlänk:
Sammanfattning (abstract):
Here, we aimed to investigate potential effects of ocean acidification on antimicrobial peptide (AMP) activity in the gills of Mytilus edulis, as gills are directly facing seawater and the changing pH (predicted to be reduced from ∼8.1 to ∼7.7 by 2100). The AMP activity of gill and haemocyte extracts was compared at pH 6.0, 7.7 and 8.1, with a radial diffusion assay against Escherichia coli. The activity of the gill extracts was not affected by pH, while it was significantly reduced with increasing pH in the haemocyte extracts. Gill extracts were also tested against different species of Vibrio (V. parahaemolyticus, V. tubiashii, V. splendidus, V. alginolyticus) at pH 7.7 and 8.1. The metabolic activity of the bacteria decreased by ∼65–90%, depending on species of bacteria, but was, as in the radial diffusion assay, not affected by pH. The results indicated that AMPs from gills are efficient in a broad pH-range. However, when mussels were pre-exposed for pH 7.7 for four month the gill extracts presented significantly lower inhibit of bacterial growth. A full in-depth proteome investigation of gill extracts, using LC-Orbitrap MS/MS technique, showed that among previously described AMPs from haemocytes of Mytilus, myticin A was found up-regulated in response to lipopolysaccharide, 3 h post injection. Sporadic occurrence of other immune related peptides/proteins also pointed to a rapid response (0.5–3 h p.i.). Altogether, our results indicate that the gills of blue mussels constitute an important first line defence adapted to act at the pH of seawater. The antimicrobial activity of the gills is however modulated when mussels are under the pressure of ocean acidification, which may give future advantages for invading pathogens.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
NATURVETENSKAP ->
Biologiska vetenskaper
Nyckelord:
Ocean acidification; Mytilus edulis; Antimicrobial peptide; Gill tissue; Vibrio; LPS; Proteome analysis; LC-Orbitrap MS/MS analysis
Postens nummer:
235145
Posten skapad:
2016-04-25 11:10
Posten ändrad:
2016-07-05 10:13

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