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Göteborgs universitets publikationer

Quantification of heterotypic granule fusion in human neutrophils by imaging flow cytometry.

Författare och institution:
Halla Björnsdottir (Institutionen för odontologi); Amanda Welin (Institutionen för medicin, avdelningen för reumatologi och inflammationsforskning); Claes Dahlgren (Institutionen för medicin, avdelningen för reumatologi och inflammationsforskning); Anna Karlsson (Institutionen för medicin, avdelningen för reumatologi och inflammationsforskning); Johan Bylund (Institutionen för odontologi, sektion 3)
Publicerad i:
Data in brief, 6 s. 386-93
ISSN:
2352-3409
Publikationstyp:
Artikel, refereegranskad vetenskaplig
Publiceringsår:
2016
Språk:
engelska
Fulltextlänk:
Sammanfattning (abstract):
Human neutrophils are filled with intracellular storage organelles, called granules and secretory vesicles, which differ in their content of soluble matrix proteins and membrane-bound molecules. To date, at least four distinct granule/vesicle subsets have been identified. These organelles may secrete their content extracellularly following mobilization to and fusion with the plasma membrane, but some of them may also fuse with internal membrane-enclosed organelles, typically a plasma membrane-derived phagosome. There are also instances where different granules appear to fuse with one another, a process that would enable mixing of their matrix and membrane components. Such granule fusion enables e.g., myeloperoxidase-processing of intragranular oxygen radicals, a key event in the formation of neutrophil extracellular traps (Björnsdottir et al., 2015) [1]. Described herein are data that show the quantification of such heterotypic granule-granule fusion by the use of imaging flow cytometry, a technique that combines flow cytometry with microscopy. The analysis described is based on immunofluorescent staining of established granule markers (lactoferrin and/or NGAL for one granule subset; the specific granules, and CD63 for another granule subset, the azurophil granules) and calculation of a colocalization score for resting and PMA-stimulated neutrophils.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
MEDICIN OCH HÄLSOVETENSKAP ->
Annan medicin och hälsovetenskap
Postens nummer:
234648
Posten skapad:
2016-04-14 08:15
Posten ändrad:
2016-06-23 14:31

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