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Göteborgs universitets publikationer

Liver lipid metabolism is altered by increased circulating estrogen to androgen ratio in male mouse

Författare och institution:
A. P. Vehmas (-); M. Adam (-); T. D. Laajala (-); G. Kastenmuller (-); C. Prehn (-); J. Rozman (-); Claes Ohlsson (Centre for Bone and Arthritis Research); H. Fuchs (-); M. H. de Angelis (-); V. Gailus-Durner (-); L. L. Elo (-); T. Aittokallio (-); J. Adamski (-); G. Corthals (-); Matti Poutanen (Institutionen för medicin); L. Strauss (-)
Publicerad i:
Journal of Proteomics, 133 s. 66-75
Artikel, refereegranskad vetenskaplig
Sammanfattning (abstract):
Estrogens are suggested to lower the risk of developing metabolic syndrome in both sexes. In this study, we investigated how the increased circulating estrogen-to-androgen ratio (E/A) alters liver lipid metabolism in males. The cytochrome P450 aromatase (P450arom) is an enzyme converting androgens to estrogens. Male mice overexpressing human aromatase enzyme (AROM + mice), and thus have high circulating E/A, were used as a model in this study. Proteomics and gene expression analyses indicated an increase in the peroxisomal beta-oxidation in the liver of AROM + mice as compared with their wild type littermates. Correspondingly, metabolomic analysis revealed a decrease in the amount of phosphatidylcholines with long-chain fatty acids in the plasma. With interest we noted that the expression of Cyp4a12a enzyme, which specifically metabolizes arachidonic acid (AA) to 20-hydroxy AA, was dramatically decreased in the AROM + liver. As a consequence, increased amounts of phospholipids having AA as a fatty acid tail were detected in the plasma of the AROM + mice. Overall, these observations demonstrate that high circulating E/A in males is linked to indicators of higher peroxisomal beta-oxidation and lower AA metabolism in the liver. Furthermore, the plasma phospholipid profile reflects the changes in the liver lipid metabolism. (C) 2015 Elsevier B.V. All rights reserved.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Klinisk medicin ->
Endokrinologi och diabetes
Liver, Aromatase, Male mouse, Label free quantitative proteomics, Metabolomics, Phospholipid, transgenic male-mice, acyl-coa thioesterases, mass-spectrometry, ppar-alpha, glucose-homeostasis, insulin sensitivity, hepatic steatosis, protein abundance, gene-expression, aromatase gene, Biochemistry & Molecular Biology
Postens nummer:
Posten skapad:
2016-03-22 11:07

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