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Göteborgs universitets publikationer

Interaction between dietary lipids and gut microbiota regulates hepatic cholesterol metabolism

Författare och institution:
Robert Caesar (Wallenberglaboratoriet); H. Nygren (-); M. Oresic (-); Fredrik Bäckhed (Wallenberglaboratoriet)
Publicerad i:
Journal of Lipid Research, 57 ( 3 ) s. 474-481
ISSN:
0022-2275
Publikationstyp:
Artikel, refereegranskad vetenskaplig
Publiceringsår:
2016
Språk:
engelska
Fulltextlänk:
Sammanfattning (abstract):
The gut microbiota influences many aspects of host metabolism. We have previously shown that the presence of a gut microbiota remodels lipid composition. Here we investigated how interaction between gut microbiota and dietary lipids regulates lipid composition in the liver and plasma, and gene expression in the liver. Germ-free and conventionally raised mice were fed a lard or fish oil diet for 11 weeks. We performed lipidomics analysis of the liver and serum and microarray analysis of the liver. As expected, most of the variation in the lipidomics dataset was induced by the diet, and abundance of most lipid classes differed between mice fed lard and fish oil. However, the gut microbiota also affected lipid composition. The gut microbiota increased hepatic levels of cholesterol and cholesteryl esters in mice fed lard, but not in mice fed fish oil. Serum levels of cholesterol and cholesteryl esters were not affected by the gut microbiota. Genes encoding enzymes involved in cholesterol biosynthesis were downregulated by the gut microbiota in mice fed lard and were expressed at a low level in mice fed fish oil independent of microbial status. In summary, we show that gut microbiota-induced regulation of hepatic cholesterol metabolism is dependent on dietary lipid composition.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
MEDICIN OCH HÄLSOVETENSKAP ->
Klinisk medicin
Nyckelord:
cholesterol/biosynthesis, fish oil, gene expression, lipidomics, liver, microarrays, germ-free, colon-cancer cells, high-fat-diet, intestinal microbiota, mass-spectrometry, induced obesity, er stress, mice, acid, resistance, profile, Biochemistry & Molecular Biology
Postens nummer:
233395
Posten skapad:
2016-03-18 10:58
Posten ändrad:
2016-06-22 11:49

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