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Göteborgs universitets publikationer

SILAC-Based Quantitative Proteomic Analysis of Diffuse Large B-Cell Lymphoma Patients.

Författare och institution:
Ulla Rüetschi (Institutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin); Martin Stenson (Institutionen för medicin); Sverker Hasselblom (Institutionen för medicin, avdelningen för invärtesmedicin och klinisk nutrition); Herman Nilsson-Ehle (Institutionen för medicin, avdelningen för invärtesmedicin och klinisk nutrition); Ulrika Hansson (Institutionen för biomedicin); Henrik Fagman (Institutionen för biomedicin); Per-Ola Andersson (Institutionen för medicin)
Publicerad i:
International journal of proteomics, 2015 s. 841769
Artikel, refereegranskad vetenskaplig
Sammanfattning (abstract):
Diffuse large B-cell lymphoma (DLBCL), the most common lymphoma, is a heterogeneous disease where the outcome for patients with early relapse or refractory disease is very poor, even in the era of immunochemotherapy. In order to describe possible differences in global protein expression and network patterns, we performed a SILAC-based shotgun (LC-MS/MS) quantitative proteomic analysis in fresh-frozen tumor tissue from two groups of DLBCL patients with totally different clinical outcome: (i) early relapsed or refractory and (ii) long-term progression-free patients. We could identify over 3,500 proteins; more than 1,300 were quantified in all patients and 87 were significantly differentially expressed. By functional annotation analysis on the 66 proteins overexpressed in the progression-free patient group, we found an enrichment of proteins involved in the regulation and organization of the actin cytoskeleton. Also, five proteins from actin cytoskeleton regulation, applied in a supervised regression analysis, could discriminate the two patient groups. In conclusion, SILAC-based shotgun quantitative proteomic analysis appears to be a powerful tool to explore the proteome in DLBCL tumor tissue. Also, as progression-free patients had a higher expression of proteins involved in the actin cytoskeleton protein network, such a pattern indicates a functional role in the sustained response to immunochemotherapy.
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Postens nummer:
Posten skapad:
2016-03-15 15:43
Posten ändrad:
2016-06-22 11:38

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