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Göteborgs universitets publikationer

Arabidopsis COP1 SUPPRESSOR 2 Represses COP1 E3 Ubiquitin Ligase Activity through Their Coiled-Coil Domains Association

Författare och institution:
Dongqing Xu (Institutionen för biologi och miljövetenskap); F. Lin (-); Jiang Yang (Institutionen för biologi och miljövetenskap); J. J. Ling (-); C. Hettiarachchi (-); C. Tellgren-Roth (-); Magnus Holm (Institutionen för biologi och miljövetenskap); N. Wei (-); X. W. Deng (-)
Publicerad i:
Plos Genetics, 11 ( 12 ) s. artikel nr e1005747
Artikel, refereegranskad vetenskaplig
Sammanfattning (abstract):
CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1) functions as an E3 ubiquitin ligase and mediates a variety of developmental processes in Arabidopsis by targeting a number of key regulators for ubiquitination and degradation. Here, we identify a novel COP1 interacting protein, COP1 SUPPRESSOR 2 (CSU2). Loss of function mutations in CSU2 suppress the constitutive photomorphogenic phenotype of cop1-6 in darkness. CSU2 directly interacts with COP1 via their coiled-coil domains and is recruited by COP1 into nuclear speckles in living plant cells. Furthermore, CSU2 inhibits COP1 E3 ubiquitin ligase activity in vitro, and represses COP1 mediated turnover of HY5 in cell-free extracts. We propose that in csu2 cop1-6 mutants, the lack of CSU2's repression of COP1 allows the low level of COP1 to exhibit higher activity that is sufficient to prevent accumulation of HY5 in the dark, thus restoring the etiolated phenotype. In addition, CSU2 is required for primary root development under normal light growth condition.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Annan naturvetenskap
light-dependent interaction, blue-light, transcription-factor, tumor-suppressor, gene-expression, photomorphogenic development, seedling development, protein stability, plant development, cryptochrome, 1, Genetics & Heredity
Postens nummer:
Posten skapad:
2016-03-08 10:24
Posten ändrad:
2016-03-16 14:58

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