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Göteborgs universitets publikationer

Single-molecule imaging of individual amyloid protein aggregates in human biofluids.

Författare och institution:
Mathew Harry Horrocks (-); Steven F Lee (-); Sonia Gandhi (-); Nadia K Magdalinou (-); Serene W Chen (-); Michael J Devine (-); Laura Tosatto (-); Magnus Kjaergaard (-); Joseph S Beckwith (-); Henrik Zetterberg (Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi); Marija Iljina (-); Nunilo Cremades (-); Christopher M Dobson (-); Nicholas W Wood (-); David Klenerman (-)
Publicerad i:
ACS chemical neuroscience, 16;7 ( 3 ) s. 399-406
Artikel, refereegranskad vetenskaplig
Sammanfattning (abstract):
The misfolding and aggregation of proteins into amyloid fibrils characterizes many neurodegenerative disorders such as Parkinson's and Alzheimer's diseases. We report here a method, termed SAVE (single aggregate visualization by enhancement) imaging, for the ultra-sensitive detection of individual amyloid fibrils and oligomers using single-molecule fluorescence microscopy. We demonstrate that this method is able to detect the presence of amyloid aggregates of alpha-synuclein, tau and amyloid-β. In addition, we show that aggregates can also be identified in human cerebrospinal fluid (CSF). Significantly, we see a two-fold increase in the average aggregate concentration in CSF from PD patients compared to age-matched controls. Taken together, we conclude that this method provides an opportunity to characterize the structural nature of amyloid aggregates in a key biofluid, and therefore has the potential to study disease progression in both animal models and humans to enhance our understanding of neurodegenerative disorders.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Medicinska grundvetenskaper ->
Neurovetenskaper ->
Postens nummer:
Posten skapad:
2016-02-04 16:59
Posten ändrad:
2016-04-18 14:02

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