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Göteborgs universitets publikationer

Tissue Plasminogen Activator Coating on Implant Surfaces Reduces Staphylococcus aureus Biofilm Formation

Författare och institution:
Jakub Kwiecinski (Institutionen för medicin, avdelningen för reumatologi och inflammationsforskning); Manli Na (Institutionen för medicin, avdelningen för reumatologi och inflammationsforskning); Anders Jarneborn (Institutionen för medicin, avdelningen för reumatologi och inflammationsforskning); G. Jacobsson (-); M. Peetermans (-); P. Verhamme (-); Tao Jin (Institutionen för medicin, avdelningen för reumatologi och inflammationsforskning)
Publicerad i:
Applied and Environmental Microbiology, 82 ( 1 ) s. 394-401
Artikel, refereegranskad vetenskaplig
Sammanfattning (abstract):
Staphylococcus aureus biofilm infections of indwelling medical devices are a major medical challenge because of their high prevalence and antibiotic resistance. As fibrin plays an important role in S. aureus biofilm formation, we hypothesize that coating of the implant surface with fibrinolytic agents can be used as a new method of antibiofilm prophylaxis. The effect of tissue plasminogen activator (tPA) coating on S. aureus biofilm formation was tested with in vitro microplate biofilm assays and an in vivo mouse model of biofilm infection. tPA coating efficiently inhibited biofilm formation by various S. aureus strains. The effect was dependent on plasminogen activation by tPA, leading to subsequent local fibrin cleavage. A tPA coating on implant surfaces prevented both early adhesion and later biomass accumulation. Furthermore, tPA coating increased the susceptibility of biofilm infections to antibiotics. In vivo, significantly fewer bacteria were detected on the surfaces of implants coated with tPA than on control implants from mice treated with cloxacillin. Fibrinolytic coatings (e.g., with tPA) reduce S. aureus biofilm formation both in vitro and in vivo, suggesting a novel way to prevent bacterial biofilm infections of indwelling medical devices.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Medicinska grundvetenskaper ->
Mikrobiologi inom det medicinska området
Klinisk medicin ->
Reumatologi och inflammation
skin infections, disease, staphylothrombin, quantification, inhibition, severity, adhesion, strains, fibrin, Biotechnology & Applied Microbiology, Microbiology
Postens nummer:
Posten skapad:
2016-01-15 12:25

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