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Göteborgs universitets publikationer

Interferon-gamma enhances both the anti-bacterial and the pro-inflammatory response of human mast cells to Staphylococcus aureus

Författare och institution:
E. J. Swindle (-); J. M. Brown (-); Madeleine Rådinger (Krefting Research Centre); F. R. DeLeo (-); D. D. Metcalfe (-)
Publicerad i:
Immunology, 146 ( 3 ) s. 470-485
Artikel, refereegranskad vetenskaplig
Sammanfattning (abstract):
Human mast cells (huMCs) are involved in both innate and adaptive immune responses where they release mediators including amines, reactive oxygen species (ROS), eicosanoids and cytokines. We have reported that interferon- (IFN-gamma) enhances FcR-dependent ROS production. The aim of this study was to extend these observations by investigating the effect of IFN- on the biological responses of huMCs to Staphylococcus aureus. We found that exposure of huMCs to S.aureus generated intracellular and extracellular ROS, which were enhanced in the presence of IFN-gamma IFN-gamma also promoted bacteria killing, -hexosaminidase release and eicosanoid production. Interferon- similarly increased expression of mRNAs encoding CCL1 to CCL4, granulocyte-macrophage colony-stimulating factor (GM-CSF), tumour necrosis factor- and CXCL8 in S.aureus-stimulated huMCs. The ability of IFN- to increase CXCL8 and GM-CSF protein levels was confirmed by ELISA. Fibronectin or a beta(1) integrin blocking antibody completely abrogated IFN-gamma-dependent S.aureus binding and reduced S.aureus-dependent CXCL8 secretion. These data demonstrate that IFN-gamma primes huMCs for enhanced anti-bacterial and pro-inflammatory responses to S.aureus, partially mediated by beta(1) integrin.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Medicinska grundvetenskaper ->
Immunologi inom det medicinska området
bacteria, innate immunity, beta(1) integrin, interferon-gamma, mast cells, fc-epsilon-ri, necrosis-factor-alpha, escherichia-coli, ifn-gamma, tnf-alpha, epithelial-cells, innate immunity, up-regulation, in-vitro, activation
Postens nummer:
Posten skapad:
2015-11-06 11:54

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