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Göteborgs universitets publikationer

An inflammatory equine model demonstrates dynamic changes of immune response and cartilage matrix molecule degradation in vitro

Författare och institution:
Emilia Svala (Institutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin); M. Lofgren (-); Carina Sihlbom (Core Facilities, Proteomics); Ulla Rüetschi (Institutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin); Anders Lindahl (Institutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin); S. Ekman (-); Eva Skiöldebrand (Institutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin)
Publicerad i:
Connective Tissue Research, 56 ( 4 ) s. 315-325
ISSN:
0300-8207
Publikationstyp:
Artikel, refereegranskad vetenskaplig
Publiceringsår:
2015
Språk:
engelska
Fulltextlänk:
Sammanfattning (abstract):
The molecular aspects of inflammation were investigated in equine articular cartilage explants using quantitative proteomics. Articular cartilage explants were stimulated with interleukin (IL)-1 beta in vitro for 25 days, and proteins released into cell culture media were chemically labeled with isobaric mass tags and analyzed by liquid chromatography-tandem mass spectrometry. A total of 127 proteins were identified and quantified in media from explants. IL-1 beta-stimulation resulted in an abundance of proteins related to inflammation, including matrix metalloproteinases, acute phase proteins, complement components and IL-6. Extracellular matrix (ECM) molecules were released at different time points, and fragmentation of aggrecan and cartilage oligomeric matrix protein was observed at days 3 and 6, similar to early-stage OA in vivo. Degradation products of the collagenous network were observed at days 18 and 22, similar to late-stage OA. This model displays a longitudinal quantification of released molecules from the ECM of articular cartilage. Identification of dynamic changes of extracellular matrix molecules in the secretome of equine explants stimulated with IL-1 beta over time may be useful for identifying components released at different time points during the spontaneous OA process.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
MEDICIN OCH HÄLSOVETENSKAP ->
Klinisk medicin
Nyckelord:
Horse, IL-1 beta, matrix molecules, osteoarthritis, proteomics, HUMAN SYNOVIAL-FLUID, ARTICULAR-CARTILAGE, JOINT DISEASE, PROTEIN, THROMBOSPONDIN-5, INTERGLOBULAR DOMAIN, AGGRECAN FRAGMENTS, COMPLEMENT-SYSTEM, COLLAGEN-IX, OSTEOARTHRITIS, INTERLEUKIN-1-BETA
Postens nummer:
223002
Posten skapad:
2015-09-22 13:37
Posten ändrad:
2016-08-10 13:12

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