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Specificity of binding of a strain of uropathogenic Escherichia coli to Gal alpha 1----4Gal-containing glycosphingolipids.

Författare och institution:
K Bock (-); Michael Breimer (Medicinska institutionen); A Brignole (-); Gunnar C. Hansson (Medicinska institutionen); K A Karlsson (-); G Larson (-); H Leffler (-); B E Samuelsson (-); N Strömberg (-); C S Edén (-)
Publicerad i:
The Journal of biological chemistry, 260 ( 14 ) s. 8545-51
Artikel, refereegranskad vetenskaplig
Sammanfattning (abstract):
A strain of Escherichia coli originally isolated from urine of a patient with acute pyelonephritis was studied in detail for binding to glycosphingolipids. Bacteria labeled metabolically with [14C]glucose were layered over a glycolipid chromatogram and bound bacteria were detected by autoradiography. The detection was down to a few ng of glycolipid (pmol level) under these assay conditions. At a test level of 500 ng all glycolipids (more than a dozen molecular species analyzed) with Gal alpha 1----4Gal as an internal or terminal part bound the bacteria strongly while glycolipids known to lack this sequence were negative. Conformational analysis using hard sphere calculations including the exo-anomeric effect showed a bend in the saccharide chain at this disaccharide with a largely hydrophobic surface of the convex side, probably being part of the binding epitope. Mixtures of glycolipids isolated from a human ureter scraping and from urinary sediments bound bacteria in the 2- to 7-sugar interval. Thus, this infectious strain of E. coli recognizes glycolipids being present in epithelial cells lining the urinary tract.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Klinisk medicin
ABO Blood-Group System, Animals, Carbohydrate Sequence, Cattle, Chromatography, Thin Layer, Dogs, Escherichia coli, metabolism, Escherichia coli Infections, microbiology, Galactose, metabolism, Glycosphingolipids, metabolism, Humans, Kidney, metabolism, Mice, Models, Molecular, Pyelonephritis, microbiology, Rabbits, Rats, Substrate Specificity
Postens nummer:
Posten skapad:
2015-02-05 07:57

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