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Göteborgs universitets publikationer

Structures of blood group glycosphingolipids of human small intestine. A relation between the expression of fucolipids of epithelial cells and the ABO, Le and Se phenotype of the donor.

Författare och institution:
S Björk (-); Michael Breimer (Medicinska institutionen); Gunnar C. Hansson (Medicinska institutionen); K A Karlsson (-); H Leffler (-)
Publicerad i:
The Journal of biological chemistry, 262 ( 14 ) s. 6758-65
Artikel, refereegranskad vetenskaplig
Sammanfattning (abstract):
Small intestinal epithelial cells (enterocytes) were isolated from specimens obtained at operation from four human individuals with different blood group ABO, Lewis, and secretor phenotypes. The non-acid glycolipids were isolated and characterized by thin-layer chromatography, mass spectrometry, and proton NMR spectroscopy and for reactivity with monoclonal antibodies on thin-layer chromatograms. Monohexosylceramides and blood group ABH (type 1 chain) and Lewis glycolipids with 5-7 sugar residues were the major compounds present in all cases, and the expression of the major blood group glycolipids was in agreement with the ABO, Lewis, and secretor phenotype of the individual donors. Small amounts of more complex glycolipids with up to 10 sugar residues were identified by mass spectrometry in all cases. In addition, small amounts of lactotetraosylceramide, a blood group H-active triglycosylceramide with the structure of Fuc alpha 1-2Gal-Hex-Cer (where Fuc is fucose, Hex is hexose, and Cer is ceramide), and dihexosylceramides were identified in some cases. Globotriaosyl- and globotetraosylceramides were absent from the epithelial cells. Small amounts of Leb-active glycolipids in blood group OLe(a+b-), non-secretor and OLe(a-b-), secretor individuals as well as trace amounts of type 2 carbohydrate chain compounds in all individuals were detected by specific monoclonal antibodies.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Klinisk medicin
ABO Blood-Group System, Adult, Aged, Antibodies, Monoclonal, Blood Group Antigens, Carbohydrate Conformation, Carbohydrate Sequence, Epithelium, immunology, Female, Fucose, analysis, Glycolipids, isolation & purification, Glycosphingolipids, isolation & purification, Humans, Intestine, Small, immunology, Lewis Blood-Group System, Male, Mass Spectrometry, Middle Aged, Phenotype
Postens nummer:
Posten skapad:
2015-02-02 15:24

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