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Comparison of the FilmArray assay and in-house real-time PCR for detection of respiratory infection

Författare och institution:
E. M. Andersson (Institutionen för biomedicin, avdelningen för infektionssjukdomar); Sigvard Olofsson (Institutionen för biomedicin, avdelningen för infektionssjukdomar); Magnus Lindh (Institutionen för biomedicin, avdelningen för infektionssjukdomar)
Publicerad i:
Scandinavian Journal of Infectious Diseases, 46 ( 12 ) s. 897-901
Artikel, refereegranskad vetenskaplig
Sammanfattning (abstract):
Recently, molecular methods capable of detecting almost all microbial agents that may cause acute respiratory infection have been introduced. The FilmArray Respiratory Panel assay, which integrates nucleic acid extraction, nested amplification and detection in a reaction pouch preloaded with all reagents required for detection of 17 viruses and 3 bacteria, was compared with an in-house real-time PCR that detects these agents in 8 parallel amplifications. When 128 clinical samples representing 18 of these agents were analysed by both assays the agreement was excellent, with kappa values ranging between 0.54 and 1.0. Discordances were mainly observed for adenovirus, but not when version 1.7 of FilmArray was used. The results show that these assays detect a wide range of pathogens with similar performance. FilmArray provides results after approximately 1 h, including approximate to 5 min hands-on time, and does not require advanced equipment or expertise in molecular diagnostics, making it a useful point-of-care-test for acute respiratory infections.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Klinisk medicin
Acute respiratory infection, real-time PCR, point-of care testing, FilmArray, molecular diagnostics, VIRUSES, PANEL, PATHOGENS, DIAGNOSIS, Infectious Diseases
Postens nummer:
Posten skapad:
2014-12-19 15:54

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