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Göteborgs universitets publikationer

Fab-mediated binding of glycoprotein Ib/IX and IIb/IIIa specific antibodies in chronic idiopathic thrombocytopenic purpura.

Författare och institution:
M Hou (Institutionen för invärtesmedicin, Avdelningen för internmedicin); Dick Stockelberg (Institutionen för invärtesmedicin, Avdelningen för internmedicin); Jack Kutti (Institutionen för invärtesmedicin, Avdelningen för internmedicin); Hans Wadenvik (Institutionen för invärtesmedicin, Avdelningen för internmedicin)
Publicerad i:
British journal of haematology, 91 ( 4 ) s. 944-50
Artikel, refereegranskad vetenskaplig
Sammanfattning (abstract):
The antibody domain responsible for the interactions between platelet glycoproteins (GP) and serum IgG autoantibodies in patients with chronic idiopathic thrombocytopenic purpura (ITP) was studied. Sera from nine non-transfused ITP patients and 20 normal controls and a serum containing an anti-PlA1 antibody were employed. Serum, purified IgG and F(ab')2 fragments were prepared and their binding to platelet GPIb/IX and GPIIb/IIIa were analysed using a modified MAIPA assay and an antigen capture ELISA. In all experiments most of the autoantibodies studied behaved identically to the anti-PlA1 antibody in that the IgG-F(ab')2 fragments retained their ability to bind to the respective glycoprotein. Substituting the enzyme-conjugated secondary antibody (Fab specific), in the MAIPA assay, with an Fc specific antibody removed all reactivities observed against platelet GPs, produced by IgG-F(ab')2 fragments. Furthermore, in an antigen-capture ELISA, IgG autoantibodies against platelet GPIb/IX and/or GPIIb/IIIa were blocked preferentially by pre-incubating the ITP sera with a goat anti-human IgG (F(ab')2 specific) antibody, but not with an anti-Fc antibody. We conclude that these ITP patients produced antibodies specific for platelet GPIb/IX and/or GPIIb/IIIa, and that the autoantibody-platelet interaction was mediated by the classic Fab binding.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Klinisk medicin
Adult, Aged, Aged, 80 and over, Autoantibodies, metabolism, Blood Platelets, metabolism, Chronic Disease, Electrophoresis, Polyacrylamide Gel, Enzyme-Linked Immunosorbent Assay, Female, Humans, Immunoglobulin Fab Fragments, isolation & purification, metabolism, Immunoglobulin G, isolation & purification, Immunologic Techniques, Male, Middle Aged, Platelet Glycoprotein GPIIb-IIIa Complex, metabolism, Platelet Glycoprotein GPIb-IX Complex, metabolism, Protein Binding, Purpura, Thrombocytopenic, Idiopathic, immunology, metabolism
Postens nummer:
Posten skapad:
2014-05-09 16:50

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