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Göteborgs universitets publikationer

Sub-Typing of Extended-Spectrum-beta-Lactamase-Producing Isolates from a Nosocomial Outbreak: Application of a 10-Loci Generic Escherichia coli Multi-Locus Variable Number Tandem Repeat Analysis

Författare och institution:
Nahid Karami (Institutionen för biomedicin, avdelningen för infektionssjukdomar); Lisa Helldal (Institutionen för biomedicin, avdelningen för infektionssjukdomar); Christina Welinder-Olsson (Institutionen för biomedicin, avdelningen för infektionssjukdomar); Christina Åhrén (Institutionen för biomedicin, avdelningen för infektionssjukdomar); Edward R.B. Moore (Institutionen för biomedicin, avdelningen för infektionssjukdomar)
Publicerad i:
Plos One, 8 ( 12 ) s. e83030
ISSN:
1932-6203
Publikationstyp:
Artikel, refereegranskad vetenskaplig
Publiceringsår:
2013
Språk:
engelska
Fulltextlänk:
Sammanfattning (abstract):
Extended-spectrum beta-lactamase producing Escherichia coli (ESBL-E. coli) were isolated from infants hospitalized in a neonatal, post-surgery ward during a four-month-long nosocomial outbreak and six-month follow-up period. A multi-locus variable number tandem repeat analysis (MLVA), using 10 loci (GECM-10), for 'generic' (i.e., non-STEC) E. coli was applied for sub-species-level (i.e., sub-typing) delineation and characterization of the bacterial isolates. Ten distinct GECM-10 types were detected among 50 isolates, correlating with the types defined by pulsed-field gel electrophoresis (PFGE), which is recognized to be the 'gold-standard' method for clinical epidemiological analyses. Multi-locus sequence typing (MLST), multiplex PCR genotyping of bla(CTX-M), bla(TEM), bla(OXA) and bla(SHV) genes and antibiotic resistance profiling, as well as a PCR assay specific for detecting isolates of the pandemic O25b-ST131 strain, further characterized the outbreak isolates. Two clusters of isolates with distinct GECM-10 types (G06-04 and G07-02), corresponding to two major PFGE types and the MLST-based sequence types (STs) 131 and 1444, respectively, were confirmed to be responsible for the outbreak. The application of GECM-10 sub-typing provided reliable, rapid and cost-effective epidemiological characterizations of the ESBL-producing isolates from a nosocomial outbreak that correlated with and may be used to replace the laborious PFGE protocol for analyzing generic E. coli.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
NATURVETENSKAP ->
Biologiska vetenskaper ->
Mikrobiologi
MEDICIN OCH HÄLSOVETENSKAP ->
Klinisk medicin ->
Infektionsmedicin
Nyckelord:
FIELD GEL-ELECTROPHORESIS, RAPID DETECTION, ENTEROBACTERIACEAE, STRAINS, CLONE, EPIDEMIOLOGY, COLLECTION, HOSPITALS, INFECTION
Postens nummer:
193537
Posten skapad:
2014-02-06 13:06
Posten ändrad:
2016-05-04 14:15

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