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Göteborgs universitets publikationer

Composition Based Strategies for Controlling Radii in Lipid Nanotubes

Författare och institution:
Michael E. Kurczy (Institutionen för kemi- och bioteknik, Analytisk kemi, Chalmers); Lisa J. Mellander (Institutionen för kemi och molekylärbiologi); Neda Najafinobar (Institutionen för kemi- och bioteknik, Analytisk kemi, Chalmers); Ann-Sofie Cans (Institutionen för kemi- och bioteknik, Analytisk kemi, Chalmers)
Publicerad i:
PLoS ONE, 9 ( 1 ) s. e81293
Artikel, refereegranskad vetenskaplig
Fulltextlänk (lokalt arkiv):
Sammanfattning (abstract):
Nature routinely carries out small-scale chemistry within lipid bound cells and organelles. Liposome–lipid nanotube networks are being developed by many researchers in attempt to imitate these membrane enclosed environments, with the goal to perform small-scale chemical studies. These systems are well characterized in terms of the diameter of the giant unilamellar vesicles they are constructed from and the length of the nanotubes connecting them. Here we evaluate two methods based on intrinsic curvature for adjusting the diameter of the nanotube, an aspect of the network that has not previously been controllable. This was done by altering the lipid composition of the network membrane with two different approaches. In the first, the composition of the membrane was altered via lipid incubation of exogenous lipids; either with the addition of the low intrinsic curvature lipid soy phosphatidylcholine (soy-PC) or the high intrinsic curvature lipid soy phosphatidylethanolamine (soy-PE). In the second approach, exogenous lipids were added to the total lipid composition during liposome formation. Here we show that for both lipid augmentation methods, we observed a decrease in nanotube diameter following soy-PE additions but no significant change in size following the addition of soy-PC. Our results demonstrate that the effect of soy-PE on nanotube diameter is independent of the method of addition and suggests that high curvature soy-PE molecules facilitate tube membrane curvature.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Bending, Curvature, Lipids, Liposomes, Membrane characteristics, Nanotubes, Radii, Vesicles
Postens nummer:
Ingår i post nr:
Posten skapad:
2014-01-07 12:41
Posten ändrad:
2016-08-22 08:28

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