transparent gif

 

Ej inloggad.

Göteborgs universitets publikationer

Quantitative proteomics reveals regulatory differences in the chondrocyte secretome from human medial and lateral femoral condyles in osteoarthritic patients

Författare och institution:
Johan Stenberg (Institutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin); Ulla Rüetschi (Institutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin); E. Skioldebrand (Institutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin); Johan Kärrholm (Institutionen för kliniska vetenskaper, sektionen för anestesi, biomaterial och ortopedi, Avdelningen för ortopedi); Anders Lindahl (Institutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin)
Publicerad i:
Proteome Science, 11 ( 1 ) s. Article Number: UNSP 43
ISSN:
1477-5956
Publikationstyp:
Artikel, refereegranskad vetenskaplig
Publiceringsår:
2013
Språk:
engelska
Fulltextlänk:
Sammanfattning (abstract):
Background: Osteoarthritis (OA) is a destructive joint disease and there are no known biomarkers available for an early diagnosis. To identify potential disease biomarkers and gain further insight into the disease mechanisms of OA we applied quantitative proteomics with SILAC technology on the secretomes from chondrocytes of OA knees, designated as high Mankin (HM) scored secretome. A quantitative comparison was made between the secretomes of the medial and lateral femur condyle chondrocytes in the same knee since the medial femur condyle is usually more affected in OA than the lateral condyle, which was confirmed by Mankin scoring. The medial/lateral comparison was also made on the secretomes from chondrocytes taken from one individual with no clinically apparent joint-disease, designated as low Mankin (LM) scored secretome. Results: We identified 825 proteins in the HM secretome and 69 of these showed differential expression when comparing the medial and lateral femoral compartment. The LM scored femoral condyle showed early signs of OA in the medial compartment as assessed by Mankin score. We here report the identification and relative quantification of several proteins of interest for the OA disease mechanism e.g. CYTL1, DMD and STAB1 together with putative early disease markers e.g. TIMP1, PPP2CA and B2M. Conclusions: The present study reveals differences in protein abundance between medial/lateral femur condyles in OA patients. These regulatory differences expand the knowledge regarding OA disease markers and mechanisms.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
MEDICIN OCH HÄLSOVETENSKAP ->
Medicinska grundvetenskaper ->
Cell- och molekylärbiologi
MEDICIN OCH HÄLSOVETENSKAP ->
Klinisk medicin ->
Ortopedi
Nyckelord:
Secretome, SILAC, Chondrocyte, Osteoarthritis, Proteomics, HUMAN ARTICULAR CHONDROCYTES, ENDOTHELIAL-CELL MIGRATION, PROTEIN, PHOSPHATASE 2A, LARGE GENE LISTS, SIGNALING PATHWAY, KNEE, OSTEOARTHRITIS, BIOCHEMICAL MARKER, SYNOVIAL-FLUIDS, CARTILAGE, IDENTIFICATION
Postens nummer:
186622
Posten skapad:
2013-11-13 15:02
Posten ändrad:
2016-08-10 11:56

Visa i Endnote-format

Göteborgs universitet • Tel. 031-786 0000
© Göteborgs universitet 2007