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Göteborgs universitets publikationer

Time-of-Flight Secondary Ion Mass Spectrometry Based Molecular Histology of Human Spinal Cord Tissue and Motor Neurons

Författare och institution:
Jörg Hanrieder (Institutionen för kemi- och bioteknik, Analytisk kemi, Chalmers); Per Malmberg (Institutionen för kemi och molekylärbiologi); Olle R Lindberg (Institutionen för neurovetenskap och fysiologi); John S. Fletcher (Institutionen för kemi och molekylärbiologi); Andrew G Ewing (Institutionen för kemi- och bioteknik, Analytisk kemi, Chalmers & Institutionen för kemi och molekylärbiologi)
Publicerad i:
Analytical Chemistry, 85 ( 18 ) s. 8741–8748
Artikel, refereegranskad vetenskaplig
Sammanfattning (abstract):
Secondary ion mass spectrometry is a powerful method for imaging biological samples with high spatial resolution. Whole section time-of-flight-secondary ion mass spectrometry (TOF-SIMS) scans and multivariate data analysis have been performed on the human spinal cord in order to delineate anatomical regions of interest based on their chemical distribution pattern. TOF-SIMS analysis of thoracic spinal cord sections was performed at 5 μm resolution within 2 h. Multivariate image analysis by means of principal component analysis and maximum auto correlation factor analysis resulted in detection of more than 400 m/z peaks that were found to be significantly changed. Here, the results show characteristic biochemical distributions that are well in line with major histological regions, including gray and white matter. As an approach for iterative segmentation, we further evaluated previously outlined regions of interest as identified by multivariate image analysis. Here, further discrimination of the gray matter into ventral, lateral, and dorsal neuroanatomical regions was observed. TOF-SIMS imaging has been carried out at submicrometer resolution obtaining localization and characterization of spinal motor neurons based on their chemical fingerprint, including neurotransmitter precursors that serve as molecular indicators for motor neuron integrity. Thus, TOF-SIMS can be used as an approach for chemical histology and pathology. TOF-SIMS holds immense potential for investigating the subcellular mechanisms underlying spinal cord related diseases including chronic pain and amyotrophic lateral sclerosis.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Kemi ->
Analytisk kemi
Postens nummer:
Posten skapad:
2013-11-12 09:10
Posten ändrad:
2015-06-12 13:21

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