transparent gif


Ej inloggad.

Göteborgs universitets publikationer

The PapG-adhesin at the tip of P-fimbriae provides Escherichia coli with a competitive edge in experimental bladder infections of cynomolgus monkeys.

Författare och institution:
J Winberg (-); R Möllby (-); Jörgen Bergström (Institutionen för medicinsk och fysiologisk kemi); Karl-Anders Karlsson (Institutionen för medicinsk och fysiologisk kemi); Irene Leonardsson (Institutionen för medicinsk och fysiologisk kemi); Maan Abul Milh (Institutionen för medicinsk och fysiologisk kemi); Susann Teneberg (Institutionen för medicinsk och fysiologisk kemi); D Haslam (-); B I Marklund (-); S Normark (-)
Publicerad i:
The Journal of experimental medicine, 182 ( 6 ) s. 1695-702
Artikel, refereegranskad vetenskaplig
Sammanfattning (abstract):
Human urinary tract infection is an infectious disease that depends on a series of host-microbial interactions. The bacteria first colonize the colon and then the periurethral/vaginal areas; they ascend to and infect first the bladder and then the kidneys. Expression of Escherichia coli P-fimbriae constitutes the strongest correlation to renal pathogenicity, but is also related to first-time cystitis in children. The role of P-fimbriae in the preceding steps in the infectious process is unknown. To examine this, we constructed, from a P-fimbriated E. coli strain with a class II G-adhesin preferentially binding to globoside, one isogenic mutant lacking the G-adhesin and another isogenic mutant in which we replaced the papG class II allele with a class III adhesin preferentially binding to the Forssman antigen. We report here the comparison of the adhesin knockout mutant (DS17-8) and the class-switch mutant (DS17-1) with the wild-type (DS17) for in vivo colonization of the gut, vagina, and bladder of cynomolgus monkeys. It was recently shown that the class II tip G-adhesin is a prerequisite for acute pyelonephritis to occur in the monkey model in the absence of other kidney-specific adhesins or obstruction of the urinary flow. Here we show that it is not required for bladder infection but gives a competitive advantage in mixed infections. In the vagina and colon, the G-adhesin gives no competitive advantage.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Medicinska grundvetenskaper ->
Annan medicinsk grundvetenskap ->
Adhesins, Escherichia coli, physiology, Animals, Bacterial Adhesion, DNA Primers, chemistry, Escherichia coli Infections, microbiology, Female, Fimbriae Proteins, Fimbriae, Bacterial, physiology, Intestines, microbiology, Macaca fascicularis, Mutagenesis, Site-Directed, Structure-Activity Relationship, Urinary Bladder, microbiology, Vagina, microbiology
Postens nummer:
Posten skapad:
2013-09-30 16:12

Visa i Endnote-format

Göteborgs universitet • Tel. 031-786 0000
© Göteborgs universitet 2007