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Vitamin D enhances IL-1β secretion and restricts growth of Mycobacterium tuberculosis in macrophages from TB patients

Författare och institution:
Daniel Eklund (-); Hans Lennart Persson (-); Marie Larsson (-); Amanda Welin (Institutionen för medicin, avdelningen för reumatologi och inflammationsforskning); Jonna Idh (-); Jakob Paues (-); Sven-Göran Fransson (-); Olle Stendahl (-); Thomas Schön (-); Maria Lerm (-)
Publicerad i:
International Journal of Mycobacteriology, 2 ( 1 ) s. 18-25
Artikel, refereegranskad vetenskaplig
Sammanfattning (abstract):
The emergence of multidrug-resistant strains of Mycobacterium tuberculosis (MTB), the bacterium responsible for tuberculosis (TB), has rekindled the interest in the role of nutritional supplementation of micronutrients, such as vitamin D, as adjuvant treatment. Here, the growth of virulent MTB in macrophages obtained from the peripheral blood of patients with and without TB was studied. The H37Rv strain genetically modified to express Vibrio harveyi luciferase was used to determine the growth of MTB by luminometry in the human monocyte-derived macrophages (hMDMs) from study subjects. Determination of cytokine levels in culture supernatants was performed using a flow cytometry-based bead array technique. No differences in intracellular growth of MTB were observed between the different study groups. However, stimulation with 100 nM 1,25-dihydroxyvitamin D significantly enhanced the capacity of hMDMs isolated from TB patients to control the infection. This effect was not observed in hMDMs from the other groups. The interleukin (IL)-1β and IL-10 release by hMDMs was clearly increased upon stimulation with 1,25-dihydroxyvitamin D. Furthermore, the 1,25-dihydroxyvitamin D stimulation also led to elevated levels of TNF-α (tumor necrosis factor-alpha) and IL-12p40. It was concluded that vitamin D triggers an inflammatory response in human macrophages with enhanced secretion of cytokines, as well as enhancing the capacity of hMDMs from patients with active TB to restrict mycobacterial growth.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Medicinska grundvetenskaper ->
Mikrobiologi inom det medicinska området ->
Mikrobiologi ->
Medicinsk mikrobiologi
Medicinska grundvetenskaper ->
Immunologi inom det medicinska området ->
Vitamin D, Human macrophages, Intracellular growth, TB patients, IL-1β
Postens nummer:
Posten skapad:
2013-09-11 08:48

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