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Site-specific O-glycosylation on the MUC2 mucin protein inhibits cleavage by the Porphyromonas gingivalis secreted cysteine protease (RgpB).

Författare och institution:
Sjoerd van der Post (Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi); Durai B Subramani (Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi); Malin Bäckström (Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi); Malin E V Johansson (Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi); Malene B Vester-Christensen (-); Ulla Mandel (-); Eric P Bennett (-); Henrik Clausen (-); Gunnar Dahlén (Institutionen för odontologi); Aneta Sroka (-); Jan Potempa (-); Gunnar C. Hansson (Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi)
Publicerad i:
The Journal of biological chemistry, 288 ( 20 ) s. 14636-46
ISSN:
1083-351X
Publikationstyp:
Artikel, refereegranskad vetenskaplig
Publiceringsår:
2013
Språk:
engelska
Fulltextlänk:
Sammanfattning (abstract):
The colonic epithelial surface is protected by an inner mucus layer that the commensal microflora cannot penetrate. We previously demonstrated that Entamoeba histolytica secretes a protease capable of dissolving this layer that is required for parasite penetration. Here, we asked whether there are bacteria that can secrete similar proteases. We screened bacterial culture supernatants for such activity using recombinant fragments of the MUC2 mucin, the major structural component, and the only gel-forming mucin in the colonic mucus. MUC2 has two central heavily O-glycosylated mucin domains that are protease-resistant and has cysteine-rich N and C termini responsible for polymerization. Culture supernatants of Porphyromonas gingivalis, a bacterium that secretes proteases responsible for periodontitis, cleaved the MUC2 C-terminal region, whereas the N-terminal region was unaffected. The active enzyme was isolated and identified as Arg-gingipain B (RgpB). Two cleavage sites were localized to IR↓TT and NR↓QA. IR↓TT cleavage will disrupt the MUC2 polymers. Because this site has two potential O-glycosylation sites, we tested whether recombinant GalNAc-transferases (GalNAc-Ts) could glycosylate a synthetic peptide covering the IRTT sequence. Only GalNAc-T3 was able to glycosylate the second Thr in IRTT, rendering the sequence resistant to cleavage by RgpB. Furthermore, when GalNAc-T3 was expressed in CHO cells expressing the MUC2 C terminus, the second threonine was glycosylated, and the protein became resistant to RgpB cleavage. These findings suggest that bacteria can produce proteases capable of dissolving the inner protective mucus layer by specific cleavages in the MUC2 mucin and that this cleavage can be modulated by site-specific O-glycosylation.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
MEDICIN OCH HÄLSOVETENSKAP ->
Medicinska grundvetenskaper
Nyckelord:
Adhesins, Bacterial, metabolism, Amino Acid Sequence, Animals, CHO Cells, Chromatography, Colitis, microbiology, Colon, metabolism, Cricetinae, Cysteine Endopeptidases, metabolism, Epithelium, metabolism, Glycosylation, Humans, Mass Spectrometry, Molecular Sequence Data, Mucin-2, metabolism, Porphyromonas gingivalis, enzymology
Postens nummer:
181313
Posten skapad:
2013-08-13 17:41
Posten ändrad:
2016-09-01 11:07

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