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Göteborgs universitets publikationer

Two-photon fluorescence correlation spectroscopy as a tool for measuring molecular diffusion within human skin

Författare och institution:
Stina Guldbrand (Institutionen för fysik (GU)); Vladimir Kirejev (Institutionen för fysik (GU)); Carl Simonsson (Institutionen för kemi och molekylärbiologi); Mattias Goksör (Institutionen för fysik (GU)); Maria Smedh (Core Facilities, Centre for Cellular Imaging); Marica B Ericson (Institutionen för fysik (GU))
Publicerad i:
European Journal of Pharmaceutics and Biopharmaceutics, 84 ( 2 ) s. 430-436
Artikel, refereegranskad vetenskaplig
Sammanfattning (abstract):
There is a need for tools enabling quantitative imaging of biological tissue for pharmaceutical applications. In this study, two-photon fluorescence microscopy (TPM) has been combined with fluorescence correlation spectroscopy (FCS), demonstrating proof-of-principle providing quantitative data of fluorophore concentration and diffusion in human skin. Measurements were performed on excised skin exposed to either rhodamine B (RB) or rhodamine B isothiocyanate (RBITC), chosen based on their similarity in fluorescence yield and molecular weight, but difference in chemical reactivity. The measurements were performed at tissue depths in the range 0 and 20 pm, and the diffusion coefficients at skin depths 5 and 10 mu m were found to be significantly different (P < 0.05). Overall median values for the diffusion coefficients were found to be 4.0 x 10(-13) m(2)/s and 2.0 x 10(-13) m(2)/s for RB and RBITC, respectively. These values correspond to the diffusion of a hard sphere with a volume eight times larger for RBITC compared to RB. This indicates that the RBITC have bound to biomolecules in the skin, and the measured signal is obtained from the RBITC-biomolecule complexes, demonstrating the potential of the TPM-FCS method to track molecular interactions in an intricate biological matrix such as human skin.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Medicinska grundvetenskaper ->
Farmaceutisk vetenskap
Medicinska grundvetenskaper ->
Farmaceutisk vetenskap ->
Two-photon fluorescence microscopy, Fluorescence correlation spectroscopy, Diffusion coefficient, living cells, microscopy, visualization, transport
Ytterligare information:
Biological Barriers — A Need for Novel Tools in Nanotoxicology and Nanomedicine. The 8th International Conference and Workshop - Biological Barriers - in vitro Tools in Nanotoxicology and Nanomedicine
Postens nummer:
Posten skapad:
2013-08-12 14:15
Posten ändrad:
2015-02-04 11:42

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