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Göteborgs universitets publikationer

Supragingival plaque microbial analysis in reflection to caries experience.

Författare och institution:
Alaa Mannaa (Institutionen för odontologi); Anette Carlén (Institutionen för odontologi); Guglielmo Campus (-); Peter Lingström (Institutionen för odontologi)
Publicerad i:
BMC oral health, 13 s. 5
Artikel, refereegranskad vetenskaplig
Fulltextlänk (lokalt arkiv):
Sammanfattning (abstract):
ABSTRACT: BACKGROUND: Dental caries develops as a result of the metabolism of carbohydrates by cariogenic bacteria present in a complex biofilm. The present study aimed to examine if bacteria in pooled supragingival plaque samples quantified using a "checkerboard DNA-DNA hybridization" based panel of caries-related bacteria, could reflect the caries experience in a manner similar to saliva samples analysed using a chair-side method in a previous investigation. METHODS: A total of 86 mothers and their children aged 4-6 years and 12-16 years old participated. Caries experience (DMFT/dmft; Decayed, Missing and Filled Teeth for permanent and primary teeth) was registered clinically and radiographically. Caries was recorded at the D3 level (caries into dentine). The D/d component was divided into three categories. A pooled supragingival plaque sample per participant was obtained from posterior approximal sites. Analyses of 15 bacterial species were performed using the checkerboard DNA-DNA hybridisation technique. RESULTS: No significant relationships were found between the bacterial scores and DMFT/dmft nor D/d groups. CONCLUSIONS: Unlike the saliva samples and the chair-side method, interproximal pooled plaque samples analysed using the "checkerboard DNA-DNA hybridization technique" did not reveal any significant relations between the bacterial counts and the caries experience.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Klinisk medicin ->
Ytterligare information:
© 2013 Mannaa et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Postens nummer:
Posten skapad:
2013-01-30 15:08
Posten ändrad:
2013-01-30 15:08

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