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Göteborgs universitets publikationer

Experimental evidence showing that no mitotically active female germline progenitors exist in postnatal mouse ovaries

Författare och institution:
Hua Zhang (Institutionen för kemi och molekylärbiologi); Wenjing Zheng (Institutionen för kemi och molekylärbiologi); Yan Shen (Institutionen för kemi och molekylärbiologi); Deepak Adhikari (Institutionen för kemi och molekylärbiologi); H. Ueno (-); Kui Liu (Institutionen för kemi och molekylärbiologi)
Publicerad i:
Proceedings of the National Academy of Sciences of the United States of America, 109 ( 31 ) s. 12580-12585
Artikel, refereegranskad vetenskaplig
Sammanfattning (abstract):
It has been generally accepted for more than half a century that, in most mammalian species, oocytes cannot renew themselves in postnatal or adult life, and that the number of oocytes is already fixed in fetal or neonatal ovaries. This assumption, however, has been challenged over the past decade. In this study, we have taken an endogenous genetic approach to this question and generated a multiple fluorescent Rosa26(rbw/+); Ddx4-Cre germline reporter mouse model for in vivo and in vitro tracing of the development of female germline cell lineage. Through live cell imaging and de novo folliculogenesis experiments, we show that the Ddx4-expressing cells from postnatal mouse ovaries did not enter mitosis, nor did they contribute to oocytes during de novo folliculogenesis. Our results provide evidence that supports the traditional view that no postnatal follicular renewal occurs in mammals, and no mitotically active Ddx4-expressing female germline progenitors exist in postnatal mouse ovaries.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Biologiska vetenskaper ->
Biokemi och molekylärbiologi
multifluorescent tracing, oogonial stem cells, female germline stem cells, spermatogonial stem-cells, in-vitro, vasa, oocytes, lineage, gene, differentiation, expression, generation, homolog
Postens nummer:
Posten skapad:
2012-10-17 12:52

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