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Göteborgs universitets publikationer

Yeast Aquaglyceroporins Use the Transmembrane Core to Restrict Glycerol Transport

Författare och institution:
Cecilia Geijer (Institutionen för kemi och molekylärbiologi); Doryaneh Ahmadpour (Institutionen för kemi och molekylärbiologi); Madelene Palmgren (Institutionen för kemi och molekylärbiologi); Caroline Filipsson (Institutionen för kemi och molekylärbiologi); Dagmara Medrala Klein (Institutionen för kemi och molekylärbiologi); Markus J. Tamás (Institutionen för kemi och molekylärbiologi); Stefan Hohmann (Institutionen för kemi och molekylärbiologi); Karin Lindkvist-Petersson (Institutionen för kemi och molekylärbiologi)
Publicerad i:
Journal of Biological Chemistry, 287 ( 28 ) s. 23562-23570
Artikel, refereegranskad vetenskaplig
Sammanfattning (abstract):
Aquaglyceroporins are transmembrane proteins belonging to the family of aquaporins, which facilitate the passage of specific uncharged solutes across membranes of cells. The yeast aquaglyceroporin Fps1 is important for osmoadaptation by regulating intracellular glycerol levels during changes in external osmolarity. Upon high osmolarity conditions, yeast accumulates glycerol by increased production of the osmolyte and by restricting glycerol efflux through Fps1. The extended cytosolic termini of Fps1 contain short domains that are important for regulating glycerol flux through the channel. Here we show that the transmembrane core of the protein plays an equally important role. The evidence is based on results from an intragenic suppressor mutation screen and domain swapping between the regulated variant of Fps1 from Saccharomyces cerevisiae and the hyperactive Fps1 ortholog from Ashbya gossypii. This suggests a novel mechanism for regulation of glycerol flux in yeast, where the termini alone are not sufficient to restrict Fps1 transport. We propose that glycerol flux through the channel is regulated by interplay between the transmembrane helices and the termini. This mechanism enables yeast cells to fine-tune intracellular glycerol levels at a wide range of extracellular osmolarities.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Biologiska vetenskaper ->
Biokemi och molekylärbiologi
fungus ashbya-gossypii, regulatory domain, osmotic-stress, channel, fps1p, protein, selectivity, aquaporin, facilitator, mechanism, membrane, eston gm, 1991, proceedings of the national academy of sciences of the united states
Eukaryotic unicellular organism biology systems biology of the control of cell growth and proliferation (UNICELLSYS ) (EC/FP7/201142) Mer information
Postens nummer:
Posten skapad:
2012-08-09 13:55
Posten ändrad:
2013-09-10 17:19

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