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Göteborgs universitets publikationer

Cyclin dependent kinase 8 regulates mitotic commitment in fission yeast

Författare och institution:
Zsolt Szilagyi (Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi); Gabor Banyai (Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi); Marcela Davila Lopez (Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi); C. I. McInerny (-); Claes M Gustafsson (Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi)
Publicerad i:
Molecular and Cellular Biology, 32 ( 11 ) s. 2099-2109
Artikel, refereegranskad vetenskaplig
Sammanfattning (abstract):
Temporal changes in transcription programs are coupled to control of cell growth and division. We here report that Mediator, a conserved coregulator of eukaryotic transcription, is part of a regulatory pathway that controls mitotic entry in fission yeast. The Mediator subunit cyclin-dependent kinase 8 (Cdk8) phosphorylates the forkhead 2 (Fkh2) protein in a periodic manner that coincides with gene activation during mitosis. Phosphorylation prevents degradation of the Fkh2 transcription factor by the proteasome, thus ensuring cell cycle-dependent variations in Fkh2 levels. Interestingly, Cdk8-dependent phosphorylation of Fkh2 controls mitotic entry, and mitotic entry is delayed by inactivation of the Cdk8 kinase activity or mutations replacing the phosphorylated serine residues of Fkh2. In addition, mutations in Fkh2, which mimic protein phosphorylation, lead to premature mitotic entry. Therefore, Fkh2 regulates not only the onset of mitotic transcription but also the correct timing of mitotic entry via effects on the Weel kinase. Our findings thus establish a new pathway linking the Mediator complex to control of mitotic transcription and regulation of mitotic entry in fission yeast.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Medicinsk bioteknologi
forkhead transcription factors, cell-cycle control, rna-polymerase-ii, gene-expression, schizosaccharomyces-pombe, mediator subunits, fha, domain, m-phase, fkh2p, activation
Postens nummer:
Posten skapad:
2012-06-14 16:24
Posten ändrad:
2016-04-12 12:01

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