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Göteborgs universitets publikationer

Lipid-Based Passivation in Nanofluidics

Författare och institution:
Fredrik Persson (Institutionen för fysik (GU)); J. Fritzsche (Institutionen för fysik (GU)); K. U. Mir (-); M. Modesti (-); Fredrik Westerlund (Institutionen för kemi- och bioteknik, Fysikalisk kemi, Chalmers); Jonas O. Tegenfeldt (Institutionen för fysik (GU))
Publicerad i:
Nano Letters, 12 ( 5 ) s. 2260-2265
ISSN:
1530-6984
Publikationstyp:
Artikel, refereegranskad vetenskaplig
Publiceringsår:
2012
Språk:
engelska
Fulltextlänk:
Fulltextlänk (lokalt arkiv):
Sammanfattning (abstract):
Stretching DNA in nanochannels is a useful tool for direct, visual studies of genomic DNA at the single molecule level. To facilitate the study of the interaction of linear DNA with proteins in nanochannels, we have implemented a highly effective passivation scheme based on lipid bilayers. We demonstrate virtually complete long-term passivation of nanochannel surfaces to a range of relevant reagents, including streptavidin-coated quantum dots, RecA proteins, and RecA-DNA complexes. We show that the performance of the lipid bilayer is significantly better than that of standard bovine serum albumin-based passivation. Finally, we show how the passivated devices allow us to monitor single DNA cleavage events during enzymatic degradation by DNase I. We expect that our approach will open up for detailed, systematic studies of a wide range of protein-DNA interactions with high spatial and temporal resolution.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
NATURVETENSKAP
TEKNIK OCH TEKNOLOGIER
Nyckelord:
Nanofluidics; passivation; antifouling; lipid bilayer; protein−DNA interactions; single molecules
Chalmers styrkeområden:
Livsvetenskaper
Nanovetenskap och nanoteknik
Postens nummer:
158573
Posten skapad:
2012-06-05 15:36
Posten ändrad:
2016-05-18 08:38

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