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Göteborgs universitets publikationer

Characteristics of third-generation glucose biosensors based on Corynascus thermophilus cellobiose dehydrogenase immobilized on commercially available screen-printed electrodes working under physiological conditions

Författare och institution:
Muhammad Nadeem Zafar (-); Gulnara Safina (Institutionen för kemi och molekylärbiologi); Roland Ludwig (-); Lo Gorton (-)
Publicerad i:
Analytical Biochemistry, 425 ( 1 ) s. 36-42
Artikel, refereegranskad vetenskaplig
Sammanfattning (abstract):
In this article, we describe a third-generation amperometric glucose biosensor working under physiological conditions. This glucose biosensor consists of a recently discovered cellobiose dehydrogenase from the ascomycete Corynascus thermophilus (CtCDH) immobilized on different commercially available screen-printed electrodes made of carbon (SPCEs), carboxyl-functionalized single-walled carbon nanotubes (SPCE-SWCNTs), or multiwalled carbon nanotubes (SPCE-MWCNTs) by simple physical adsorption or a combination of adsorption followed by cross-linking using poly(ethyleneglycol) (400) diglycidyl ether (PEGDGE) or glutaraldehyde (GA). The CtCDH-based third-generation glucose biosensor has a linear range between 0.025 and 30 mM and a detection limit of 10 mu M glucose. Biosensors based on SWCNTs showed a higher sensitivity and catalytic response than the ones functionalized with MWCNTs and the SPCEs. A drastic increase in response was observed for all three electrodes when the adsorbed enzyme was cross-linked with PEGDGE or GA. The operational stability of the biosensor was tested for 7 h by repeated injections of 50 mM glucose, and only a slight decrease in the electrochemical response was found. The selectivity of the CtCDH-based biosensor was tested on other potentially interfering carbohydrates such as mannose, galactose, sucrose, and fucose that might be present in blood. No significant analytical response from any of these compounds was observed. (C) 2012 Elsevier Inc. All rights reserved.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Medicinsk bioteknologi ->
Medicinsk bioteknologi (med inriktning mot cellbiologi)
Amperometric glucose sensor; Interdomain electron transfer; Haem; Screen-printed electrodes; Carbon nanotubes; Cross-linking
Postens nummer:
Posten skapad:
2012-06-01 12:00

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