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A non-peptide receptor inhibitor with selectivity for one of the neutrophil formyl peptide receptors, FPR 1.

Författare och institution:
Hülya Çevik Aras (Institutionen för medicin, avdelningen för reumatologi och inflammationsforskning); Christina Kalderén (-); Annika Jenmalm Jensen (-); Tudor I Oprea (Institutionen för medicin, avdelningen för reumatologi och inflammationsforskning); Claes Dahlgren (Institutionen för medicin, avdelningen för reumatologi och inflammationsforskning); Huamei Forsman (Institutionen för medicin, avdelningen för reumatologi och inflammationsforskning)
Publicerad i:
Biochemical pharmacology, s. Epuba head of print
Artikel, refereegranskad vetenskaplig
Sammanfattning (abstract):
The neutrophil formyl peptide receptors (FPR1 and FPR2) are members of the G-protein coupled receptor family. The signals generated by occupied FPRs are both pro-inflammatory and anti-inflammatory. Accordingly, these receptors have become a therapeutic target for the development of novel drugs that may be used to reduce injuries in inflammatory diseases including asthma, rheumatoid arthritis, Alzheimer's disease and cardiovascular diseases. To support the basis for a future pharmacological characterization, we have identified a small molecular non-peptide inhibitor with selectivity for FPR1. We used the FPR1 and FPR2 specific ligands fMLF and WKYMVM, respectively, and an earlier described ratio technique, to determine inhibitory activity combined with selectivity. We show that the compound 3,5-dichloro-N-(2-chloro-5-methyl-phenyl)-2-hydroxy-benzamide (BVT173187) fulfills the criteria for an FPR1 inhibitor selective for FPR1 over FPR2, and it inhibits the same functional repertoire in neutrophils as earlier described peptide antagonists. Accordingly, the new inhibitor reduced neutrophil activation with FPR1 agonists, leading to mobilization of adhesion molecules (CR3) and the generation of superoxide anion from the neutrophil NADPH-oxidase. The effects of a number of structural analogs were determined but these were either without activity or less active/specific than BVT173187. The potency of the new inhibitor for reduction of FPR1 activity was the same as that of the earlier described FPR1 antagonist cyclosporine H, but signaling through the C5aR and CXCR (recognizing IL8) was also affected by BVT173187.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Medicinska grundvetenskaper ->
Mikrobiologi inom det medicinska området
Postens nummer:
Posten skapad:
2012-04-12 14:43
Posten ändrad:
2016-08-30 13:21

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