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Cdk1, but not Cdk2, is the sole Cdk that is essential and sufficient to drive resumption of meiosis in mouse oocytes

Författare och institution:
Deepak Adhikari (Institutionen för kemi och molekylärbiologi); Wenjing Zheng (Institutionen för kemi och molekylärbiologi); Yan Shen (Institutionen för kemi och molekylärbiologi); Nagaraju Gorre (Institutionen för kemi och molekylärbiologi); Yao Ning (Institutionen för kemi och molekylärbiologi); Guillaume Halet (-); Philipp Kaldis (-); Kui Liu (Institutionen för kemi och molekylärbiologi)
Publicerad i:
Human Molecular Genetics, s. 1-9
Artikel, refereegranskad vetenskaplig
Sammanfattning (abstract):
Mammalian oocytes are arrested at the prophase of meiosis I during fetal or postnatal development, and the meiosis is resumed by the preovulatory surge of luteinizing hormone. The in vivo functional roles of cyclin-dependent kinases (Cdks) during the resumption of meiosis in mammalian oocytes are largely unknown. Previous studies have shown that deletions of Cdk3, Cdk4 or Cdk6 in mice result in viable animals with normal oocyte maturation, indicating that these Cdks are not essential for the meiotic maturation of oocytes. In addition, conventional knockout of Cdk1 and Cdk2 leads to embryonic lethality and postnatal follicular depletion, respectively, making it impossible to study the functions of Cdk1 and Cdk2 in oocyte meiosis. In this study, we generated conditional knockout mice with oocyte-specific deletions of Cdk1 and Cdk2. We showed that the lack of Cdk1, but not of Cdk2, leads to female infertility due to a failure of the resumption of meiosis in the oocyte. Re-introduction of Cdk1 mRNA into Cdk1-null oocytes largely resumed meiosis. Thus, Cdk1 is the sole Cdk that is essential and sufficient to drive resumption of meiosis in mouse oocytes. We also found that Cdk1 maintains the phosphorylation status of protein phosphatase 1 and lamin A/C in oocytes in order for meiosis resumption to occur.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Biologiska vetenskaper ->
Biokemi och molekylärbiologi ->
Cell- och molekylärbiologi ->
Biologiska vetenskaper ->
Ytterligare information:
HMG Advance Access published March 7, 2012 (ännu ej vol,nr, sidnr)
Postens nummer:
Posten skapad:
2012-03-22 10:42
Posten ändrad:
2012-04-03 09:06

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