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Göteborgs universitets publikationer

Ethanol-induced modulation of synaptic output from the dorsolateral striatum in rat is regulated by cholinergic interneurons.

Författare och institution:
Louise Adermark (Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi); Rhona B. C. Clarke (Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi); Bo Söderpalm (Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi); Mia Ericson (Institutionen för neurovetenskap och fysiologi, sektionen för psykiatri och neurokemi)
Publicerad i:
Neurochemistry international, 58 ( 6 ) s. 693-9
Artikel, refereegranskad vetenskaplig
Sammanfattning (abstract):
The striatum is the largest input nucleus to the basal ganglia and associated with reward-based behavior. We assessed whether acute ethanol (EtOH) exposure could modulate synaptic efficacy in the dorsolateral striatum of juvenile Wistar rats. Since acute EtOH administration can both increase and decrease the probability of release of different neurotransmitters from synaptic terminals, we used field potential recordings to evaluate the net effect of EtOH on striatal output. We showed that 50mM EtOH but not 20, 80 or 100mM, depresses population spike (PS) amplitude in the dorsolateral striatum. This depression of synaptic output is insensitive to the N-methyl-d-aspartic acid (NMDA) receptor inhibitor DL-2-amino-5-phosphonopentanoic acid (AP-5, 50μM), but is blocked in slices treated with glycine receptor antagonists (strychnine, 1μM; PMBA, 50μM), nicotinic acetylcholine receptor antagonists (mecamylamine, 10μM; methyllycaconitine citrate (MLA), 40nM), or GABA(A) receptor inhibitors (picrotoxin, 100μM; bicuculline, 2μM, 20μM). A long-term facilitation of synaptic output, which is more pronounced in slices from adult Wistar rats, is detected following EtOH washout (50, 80, 100mM). This long-term enhancement of PS amplitude is regulated by cholinergic interneurons and completely blocked by mecamylamine, MLA or the non-selective muscarinic antagonist scopolamine (10μM). Administration of 100mM EtOH significantly depresses PS amplitude in scopolamine-treated slices, suggesting that EtOH exerts dual actions on striatal output that are initiated instantly upon drug wash-on. In conclusion, EtOH modulates striatal microcircuitry and neurotransmission in a way that could be of importance for understanding the intoxicating properties as well as the acute reward sensation of EtOH.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Klinisk medicin ->
Animals, Corpus Striatum, drug effects, physiology, Ethanol, pharmacology, Female, Interneurons, physiology, Male, Rats, Rats, Wistar, Receptors, Cholinergic, metabolism, Synapses, drug effects, metabolism
Postens nummer:
Posten skapad:
2011-08-18 09:03

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