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Göteborgs universitets publikationer

Improved microbiological techniques using the polymerase chain reaction and pulsed-field gel electrophoresis for diagnosis and follow-up of enterohaemorrhagic Escherichia coli infection.

Författare och institution:
Christina Welinder-Olsson (Institutionen för laboratoriemedicin, Avdelningen för klinisk bakteriologi); Eva Kjellin (Institutionen för laboratoriemedicin, Avdelningen för klinisk bakteriologi); Maria Badenfors (Institutionen för laboratoriemedicin, Avdelningen för klinisk bakteriologi); Bertil Kaijser (Institutionen för laboratoriemedicin, Avdelningen för klinisk bakteriologi)
Publicerad i:
European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 19 ( 11 ) s. 843-51
Artikel, refereegranskad vetenskaplig
Sammanfattning (abstract):
The aims of the present investigation were to evaluate the microbiological diagnostic procedures, especially polymerase chain reaction (PCR) versus culture and seroagglutination, in relation to the clinical features of enterohaemorrhagic Escherichia coli (EHEC) infection and to study the status of EHEC in the western part of Sweden. During 1997 and 1998, stool specimens from 3,948 patients were analysed by PCR for the presence of EHEC with verotoxin (VT)1- and/or VT2-producing DNA sequences. The stool specimens were also cultured for Escherichia coli O157:H7, Salmonella, Campylobacter, Shigella and Yersinia. Fifty-five patients were positive by PCR. Thirty-nine patients were positive for EHEC by PCR and culture. Of these, 29 were infected with EHEC serogroup O157:H7 strains. All EHEC isolates were analysed by pulsed-field gel electrophoresis (PFGE); 17 different clones were identified. Studies on the duration of the presence of EHEC in the gut showed that EHEC often disappears rather quickly, i.e. within 2 weeks. In one patient, however, EHEC remained for several months. In conclusion, PCR, rather than culture and agglutination, should be the method of choice for microbiological diagnosis of EHEC infection. PCR is more sensitive than culture for detecting EHEC in the gut.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Medicinska grundvetenskaper ->
Mikrobiologi inom det medicinska området ->
Mikrobiologi ->
Klinisk bakteriologi
Adhesins, Bacterial, Adolescent, Adult, Bacterial Outer Membrane Proteins, genetics, metabolism, Bacterial Typing Techniques, Carrier Proteins, Child, Culture Media, Electrophoresis, Gel, Pulsed-Field, Escherichia coli, classification, genetics, immunology, isolation & purification, Escherichia coli Infections, diagnosis, epidemiology, microbiology, Escherichia coli Proteins, Feces, microbiology, Humans, Polymerase Chain Reaction, methods, Serotyping, Shiga Toxins, genetics, metabolism
Postens nummer:
Posten skapad:
2011-01-18 16:21
Posten ändrad:
2016-01-08 11:12

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