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Göteborgs universitets publikationer

Cardiomyocyte clusters derived from human embryonic stem cells share similarities with human heart tissue.

Författare och institution:
Julia Asp (Institutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin); Daniella Steel (-); Marianne Jonsson (Institutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin); Caroline Améen (-); Kerstin Dahlenborg (-); Anders Jeppsson (Institutionen för medicin, avdelningen för molekylär och klinisk medicin); Anders Lindahl (Institutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin); Peter Sartipy (-)
Publicerad i:
Journal of molecular cell biology, 2 ( 5 ) s. 276-83
Artikel, refereegranskad vetenskaplig
Sammanfattning (abstract):
Cardiotoxicity testing is a key activity in the pharmaceutical industry in order to detect detrimental effects of new drugs. A reliable human in vitro model would both be beneficial in selection of lead compounds and be important for reducing animal experimentation. However, the human heart is a complex organ composed of many distinct types of cardiomyocytes, but cardiomyocyte clusters (CMCs) derived from human embryonic stem cells could be an option for a cellular model. Data on functional properties of CMCs demonstrate similarities to their in vivo analogues in human. However, development of an in vitro model requires a more thorough comparison of CMCs to human heart tissue. Therefore, we directly compared individually isolated CMCs to human fetal, neonatal, adult atrial and ventricular heart tissues. Real-time qPCR analysis of mRNA levels and protein staining of ion channels and cardiac markers showed in general a similar expression pattern in CMCs and human heart. Moreover, a significant decrease in beat frequency was noted after addition of Zatebradine, a blocker to I(f) involved in regulation of spontaneous contraction in CMCs. The results underscore the similarities of CMCs to human cardiac tissue, and further support establishment of novel cardiotoxicity assays based on the CMCs in drug discovery.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Medicinska grundvetenskaper ->
Cell- och molekylärbiologi ->
Postens nummer:
Posten skapad:
2010-12-07 13:32
Posten ändrad:
2011-01-20 09:59

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