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Göteborgs universitets publikationer

A histone 3' end processing machinery in protozoa

Författare och institution:
Tore Samuelsson (Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi); Marcela Davila Lopez (Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi)
Publicerad i:
5th Annual International Conference on Intelligent Systems for Molecular Biology (ISMB) & 6th European Conference on Computational Biology (ECCB),
Konferensbidrag, poster
Sammanfattning (abstract):
In metazoa replication-dependent histone mRNAs are not polyadenylated. At the 3' end of these mRNAs there is a conserved sequence that contains a 16 nucleotide stem loop (SL) besides a purine rich sequences, called the histone downstream element (HDE) located several nucleotides downstream. This is necessary for endonucleolytic cleavage of pre-mRNA to release the mature mRNA. For this task also trans-acting factors are needed, such as a U7 snRNP, a stem loop binding protein (SLBP) and a subunit of the cleavage/polyadenylation specificity factor (CPSF-73)1,2. In order to understand the evolutionary origin of this molecular machinery we have computationally searched for all the transacting factors as well as the histone mRNA stem-loop structure in histone mRNAs in a range of eukaryotic species. We have used profile HMMs and covariance models to identify SL motifs as well as U7 RNA homologues in lower metazoa. Profile-based searches were used to identify proteins homologous to the mammalian SLBP. Surprisingly, a number of protozoan species seem to have the histone 3' end processing machinery characteristic of metazoa. These results show that this machinery is more ancient than previously anticipated.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Biologiska vetenskaper ->
Bioinformatik och systembiologi
Postens nummer:
Posten skapad:
2010-05-04 12:11
Posten ändrad:
2011-01-20 10:00

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