transparent gif


Ej inloggad.

Göteborgs universitets publikationer

Human mitochondrial transcription revisited: only TFAM and TFB2M are required for transcription of the mitochondrial genes in vitro.

Författare och institution:
Dmitry Litonin (-); Marina Sologub (-); Yonghong Shi (Institutionen för biomedicin); Maria Savkina (-); Michael Anikin (-); Maria Falkenberg (Institutionen för biomedicin); Claes M Gustafsson (Institutionen för biomedicin); Dmitry Temiakov (-)
Publicerad i:
The Journal of biological chemistry,
Artikel, refereegranskad vetenskaplig
Sammanfattning (abstract):
Human mitochondrial transcription is driven by a single subunit RNA polymerase and a set of basal transcription factors. The development of a recombinant in vitro transcription system has allowed for a detailed molecular characterization of the individual components and their contribution to transcription initiation. We found that TFAM and TFB2M act synergistically and increase transcription efficiency 100- to 200-fold compared with RNAP alone. Both the LSP and HSP1 promoters displayed maximal levels of in vitro transcription when TFAM was present in an amount equimolar to the DNA template. Importantly, we did not detect any significant transcription activity in the presence of the TFB2M paralogue, TFB1M or when templates containing the putative HSP2 promoter were used. These data confirm previous observations that TFB1M does not function as a bona fide transcription factor and raise questions as to whether HSP2 serves as a functional promoter in vivo. In addition, we did not detect transcription stimulation by the ribosomal protein MRPL12. Thus, only two essential initiation factors, TFAM and TFB2M, and two promoters, LSP and HSP1, are required to drive transcription of the mitochondrial genome.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Medicinska grundvetenskaper ->
Annan medicinsk grundvetenskap ->
DNA-protein interaction, Mitochondria, RNA polymerase, Transcription, Transcription promoter
Postens nummer:
Posten skapad:
2010-04-27 14:59
Posten ändrad:
2012-08-17 15:24

Visa i Endnote-format

Göteborgs universitet • Tel. 031-786 0000
© Göteborgs universitet 2007