Induction of protective immunity by vaccination against Chlamydia trachomatis using the major outer membrane protein adjuvanted with CpG oligodeoxynucleotide coupled to the nontoxic B subunit of cholera toxin.
Författare och institution:
Chunmei Cheng (-); Ilham Bettahi (-); Maria I Cruz-Fisher (-); Sukumar Pal (-); Pooja Jain (-); Zhenyu Jia (-); Jan Holmgren (Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi); Ali M Harandi (Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi); Luis M de la Maza (-)
Vaccine, 27 ( 44 ) s. 6239-46
Artikel, refereegranskad vetenskaplig
The present study was undertaken to test the efficacy of immunization with the native major outer membrane protein (nMOMP) of Chlamydia trachomatis mouse pneumonitis (MoPn) serovar in combination with a novel immunostimulatory adjuvant consisting of CpG oligodeoxynucleotide (ODN) linked to the nontoxic B subunit of cholera toxin (CTB-CpG) to elicit a protective immune response to C. trachomatis. High levels of Chlamydia-specific IgG antibodies were detected in the sera from BALB/c mice immunized intramuscularly and subcutaneously (i.m.+s.c.) with the nMOMP/CTB-CpG vaccine or with nMOMP adjuvanted with a mixture of CT and CpG ODN (CT+CpG). Further, these immunization schemes gave rise to significant T-cell-mediated Chlamydia-specific immune responses. No Chlamydia-specific humoral or cell-mediated immune responses were detected in the control mice vaccinated with ovalbumin together with either CTB-CpG or CT+CpG. Following an intranasal challenge with C. trachomatis the groups of mice immunized with nMOMP plus CTB-CpG, CT+CpG or live C. trachomatis were found to be protected based on their change in body weight and lung weight as well as number of inclusion forming unit recovered from the lungs, as compared with control groups immunized with ovalbumin plus either adjuvants. Interestingly, IFN-gamma-producing CD4(+), but not CD8(+), T-cells showed a significant correlation with the outcomes of the challenge. In conclusion, nMOMP in combination with the novel adjuvant CTB-CpG elicited a significant antigen-specific antibody and cell-mediated immune responses as well as protection against a pulmonary challenge with C. trachomatis.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
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Adjuvants, Immunologic, Animals, Antibodies, Bacterial, blood, Bacterial Vaccines, immunology, Body Weight, Cell Proliferation, Chlamydia Infections, immunology, prevention & control, Chlamydia trachomatis, immunology, Cholera Toxin, immunology, Female, Immunity, Cellular, Immunity, Humoral, Immunoglobulin G, blood, Lung, microbiology, pathology, Mice, Mice, Inbred BALB C, Oligodeoxyribonucleotides, immunology, Porins, immunology, Spleen, cytology, immunology, T-Lymphocytes, immunology
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