transparent gif


Ej inloggad.

Göteborgs universitets publikationer

Two glycosylation alterations of mouse intestinal mucins due to infection caused by the parasite Nippostrongylus brasiliensis.

Författare och institution:
Jessica Holmén (Institutionen för medicinsk och fysiologisk kemi); Fredrik J. Olson (Institutionen för medicinsk och fysiologisk kemi); Hasse Karlsson (Institutionen för medicinsk och fysiologisk kemi); Gunnar C. Hansson (Institutionen för medicinsk och fysiologisk kemi)
Publicerad i:
Glycoconjugate journal, 19 ( 1 ) s. 67-75
Artikel, refereegranskad vetenskaplig
Sammanfattning (abstract):
The glycosylation alterations of mouse small intestinal mucins during a 12-day infectious cycle caused by the parasite Nippostrongylus brasiliensis have been studied. The guanidinium chloride insoluble mucins were isolated at day 0 to 12 from the small intestine of infected and non-infected C57BL/6 mice. The O-linked oligosaccharides were released by reductive beta-elimination from the mucins and separated into neutral, sialylated and sulfated fractions. All fractions were analyzed by monosaccharide composition analysis and the neutral oligosaccharides were structurally characterized by gas chromatography/mass spectrometry. Two oligosaccharides containing blood group H-type epitopes (Fucalpha1-2Gal-) were transiently expressed with a maximum at day 6. Additional oligosaccharides with the common structure HexNAc-Gal-3GalNAcol were transiently induced with a maximum at day 10. Northern blot analysis on total RNA showed a transient expression at day 4-6 of the Fut2 gene encoding a Fucalpha1-2 fucosyltransferase, probably responsible for the detected blood group H-type epitopes. Comparisons with the corresponding infection in rat studied previously, revealed structurally different alterations, although occurring as transient events in both species. Both showed an induced blood group-type transferase halfway through the infection (a blood group A transferase in rat) and an induced transferase adding a terminal GalNAc (to a sialic acid- containing epitope in rat) towards the end of the infection. These differences between closely related species suggest rapid evolutionary alterations in glycosyltransferase expression.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
Medicinsk bioteknologi ->
Medicinsk bioteknologi (med inriktning mot cellbiologi)
Animals, Carbohydrate Sequence, Fucosyltransferases, genetics, metabolism, Gas Chromatography-Mass Spectrometry, Glycosylation, Intestines, metabolism, parasitology, Mice, Molecular Sequence Data, Mucins, chemistry, metabolism, N-Acetylneuraminic Acid, metabolism, Nippostrongylus, physiology, Oligosaccharides, chemistry, metabolism, RNA, Messenger, genetics, metabolism, Strongylida Infections, metabolism, parasitology, Sulfates, metabolism
Postens nummer:
Posten skapad:
2010-01-12 12:14
Posten ändrad:
2011-04-14 14:57

Visa i Endnote-format

Göteborgs universitet • Tel. 031-786 0000
© Göteborgs universitet 2007