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Göteborgs universitets publikationer

Structural dynamics of light-driven proton pumps.

Författare och institution:
Magnus Andersson (Institutionen för kemi- och bioteknik, Molekylär mikroskopi, Chalmers); Erik Malmerberg (Institutionen för kemi); Sebastian Westenhoff (Institutionen för kemi); Gergely Katona (Institutionen för kemi); Marco Cammarata (-); Annemarie Wöhri (Institutionen för kemi- och bioteknik, Molekylär mikroskopi, Chalmers); Linda C Johansson (Institutionen för kemi); Friederike Ewald (-); Mattias Eklund (-); Michael Wulff (-); Jan Davidsson (-); Richard Neutze (Institutionen för kemi)
Publicerad i:
Structure (London, England : 1993), 17 ( 9 ) s. 1265-75
ISSN:
1878-4186
Publikationstyp:
Artikel, refereegranskad vetenskaplig
Publiceringsår:
2009
Språk:
engelska
Fulltextlänk:
Sammanfattning (abstract):
Bacteriorhodopsin and proteorhodopsin are simple heptahelical proton pumps containing a retinal chromophore covalently bound to helix G via a protonated Schiff base. Following the absorption of a photon, all-trans retinal is isomerized to a 13-cis conformation, initiating a sequence of conformational changes driving vectorial proton transport. In this study we apply time-resolved wide-angle X-ray scattering to visualize in real time the helical motions associated with proton pumping by bacteriorhodopsin and proteorhodopsin. Our results establish that three conformational states are required to describe their photocycles. Significant motions of the cytoplasmic half of helix F and the extracellular half of helix C are observed prior to the primary proton transfer event, which increase in amplitude following proton transfer. These results both simplify the structural description to emerge from intermediate trapping studies of bacteriorhodopsin and reveal shared dynamical principles for proton pumping.
Ämne (baseras på Högskoleverkets indelning av forskningsämnen):
NATURVETENSKAP ->
Kemi
Postens nummer:
100599
Posten skapad:
2009-10-22 15:02
Posten ändrad:
2016-08-16 07:58

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